June 24, 2019Neurology
Human cerebral organoids are small balls of human brain cells ranging in size from a poppy seed to a small pea. Their organization, structure, and electrical signaling are similar to brain tissue. Because these cerebral organoids can survive in a controlled environment for months, nervous system diseases can be studied over time. Cerebral organoids have been used as models to study Zika virus infection, Alzheimer's disease, and Down syndrome.
According to a study published in Acta Neuropathologica Communications (14 June 2019), human skin cells have been used to create the first cerebral organoid system, or mini-brain, for studying sporadic Creutzfeldt-Jakob disease (CJD). CJD is a fatal neurodegenerative brain disease of humans believed to be caused by infectious prion protein. It affects about 1 in 1 million people. The authors hope that the human organoid model will enable them to evaluate potential therapeutics for CJD and provide greater detail about human prion disease subtypes than the rodent and nonhuman primate models currently in use.
For the study, the authors discovered how to infect five-month-old cerebral organoids with prions using samples from two patients who died of two different CJD subtypes, MV1 and MV2. Infection took about one month to confirm, and the authors monitored the organoids for changes in health indicators, such as metabolism, for more than six months. By the end of the study, the authors observed that seeding activity, an indication of infectious prion propagation, was present in all organoids exposed to the CJD samples. However, seeding was greater in organoids infected with the MV2 sample than the MV1 sample. They also reported that the MV1-infected organoids showed more damage than the MV2-infected organoids. The authors also noted other differences between how the MV1 and MV2 infections evolved in the organoids. As a result, there are now plans to further investigate those differences in hopes of identifying how different subtypes of CJD affect brain cells. Ultimately, the authors hope to learn how to prevent cell damage and to restore the function of cells damaged by prion infection. The new system also provides opportunities to test potential therapeutics for CJD in a tissue model that mimics the human brain.